Shanghai ancient flower tells about the preservation of culture medium

1) Constant temperature

After the medium is cooled, it can be stored in a refrigerator at about 10 ° C for 3 to 5 weeks; in mass production, it can be placed in an air-conditioned inoculation room, placed for about 2 weeks, and the temperature is controlled within 20 ° C; If the placement time is 2 to 5 days, the temperature should not exceed 28 °C. However, the temperature should not be too high during the placement of the medium, and the temperature should be prevented from changing greatly. As the temperature in the storage room increases or decreases, the air contained in the medium container will expand or contract. , bringing fungi into the medium, causing a large amount of contamination of the medium during storage.

2) Regular update

The medium is not suitable for long-term storage, especially solid medium. As time goes by, the water in the medium is selected in a gaseous state, and the water content of the medium is gradually reduced, so that the concentration of the medium and the physical and chemical properties are changed, and the subsequent explant culture or the subculture of the plants is transferred. And the rooting culture is unfavorable; if the medium is stored too long, the bacteria and fungi contained in the air may fall to the surface of the medium to cause pollution; when the medium containing the acetic acid, coconut milk and the like is stored, the light in the environment will Photolysis of indole acetic acid also changes some of the ingredients contained in the coconut milk. Therefore, it is necessary to prepare the medium for preparation and use in combination with the production task. Under normal circumstances, the storage time of the medium should not exceed one week.

3) Protect from light

The spare medium should be stored in a house with a dark light, because some substances such as acetic acid are easily decomposed by light. The composition of some media supplements also changes under light. Thicken the curtains in the inoculation room or cover the medium box with a thick black cloth to protect the medium from light.

4) Dustproof

The medium that has been sterilized should be protected against dust. If the culture medium is stored in a place with poor hygienic conditions, the bacteria and fungi attached to the dust will fall on the surface of the culture bottle. If the surface is not sterilized before use, it will easily enter the container with the airflow during the inoculation. Contamination, affecting the smooth progress of tissue culture, so the medium must be placed in a clean, hygienic inoculation room.

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